Spaying is a surgical procedure that removes the ovaries and uterus, which are the organs responsible for producing hormones that trigger the heat cycle. Therefore, after spaying, a cat will no longer experience the physical and behavioral changes associated with being in heat.
However, some cats may still exhibit certain behaviors that mimic heat, such as vocalizing, rubbing against objects, or acting restless. This is usually due to hormonal fluctuations or learned behavior.
If you notice your spayed cat exhibiting any of these behaviors, it's best to consult your veterinarian to rule out any underlying medical conditions.