Instructions
Hold the kitten so its back end is facing you; its tail will go up.
Use the "colon or semicolon" rule: If the animal is a male, the area below its tail should resemble a colon ( : ); if it is a female, the area should represent a semi-colon (; ).
Check to see if the urinary area and anus are close together; if so, it is a female. In male kittens, the urinary area and anus are not close together, and an undeveloped scrotum may be visible.
How to Tell If a Kitten Is Male or Female
Many times, when people want to make an addition to their home in the form of a kitten, its gender is of great importance. Perhaps there is already a male or a female in the home, or there may be breeding considerations. People also sometimes find and adopt a stray kitten without knowing its gender.