How to Treat Cat Urine on Subflooring

Most pet owners have to deal with urine stains occasionally. Cat urine, however, is very pungent and can be difficult to treat if left sitting for long periods. When this occurs, it is often necessary to treat the subflooring to prevent odors from seeping back through your carpet, linoleum and wood floors. Enzymatic pet odor eliminators have made the job much easier by breaking down the urine rather than covering up the smell. By treating your subfloors, you can completely rid your house of that cat urine smell.

Things You'll Need

  • Black light
  • Enzymatic pet odor cleaner
  • Plastic
  • Tape
  • Aluminum foil
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Instructions

    • 1

      Expose your subfloor by removing any carpet, padding or other flooring from the area. Identify the areas that need to be treated. You may notice visible stains or areas marked by a significant odor. If you cannot easily identify the trouble spots, try using a black light. This will illuminate the pet stains, ensuring you have identified all areas that need to be treated.

    • 2

      Saturate the area thoroughly with an enzymatic pet odor remover such as Nature's Miracle, Out or Feline Odor Neutralizer. These enzymatic cleaners break down the urine rather than masking its odor. This will help deter your cat from using the spot again once it is thoroughly cleaned.

    • 3

      Seal off the saturated area by covering with a layer of plastic, taped down on all sides. This will lock in the moisture to allow the enzymes to work on the proteins in the urine. Allow the area to sit for 24 hours. Remove the plastic and allow the area to dry for 24 hours. Repeat the steps as necessary until the odor is eliminated. This may require several treatments over a period of two to three weeks.

    • 4

      Allow the area to air dry naturally for several days after the enzyme cleanser treatments. This will ensure that moisture is not trapped in your subfloors once they are covered.

    • 5

      Keep your cat away from the area. Cover the area in aluminum foil, if necessary. Cats dislike the feel and sound of aluminum foil and will avoid walking on it. Over a period of a month or two, your cat will learn to avoid the area.