Are all truly black and white cats male?

No, that's a myth! There are both male and female black and white cats.

The color of a cat's fur is determined by genes, and there's no specific genetic link between color and sex. So, a black and white cat could be a male or a female.

It's possible that you've heard this myth because black and white cats are often associated with the tuxedo cat breed. However, tuxedo cats come in both male and female varieties.