Are cats with 3 colors always female?

This is a common misconception! While it's true that most calico and tortoiseshell cats are female, it's not a guarantee.

Here's why:

* The X chromosome: The genes responsible for fur color are located on the X chromosome. Female cats have two X chromosomes (XX), while males have one X and one Y chromosome (XY).

* Coat pattern inheritance: The calico and tortoiseshell patterns result from random inactivation of one of the X chromosomes in each cell. This leads to patches of different colored fur.

* Rare exceptions: It's possible for a male cat to have the calico or tortoiseshell pattern, but it's extremely rare. This happens when a male cat inherits an extra X chromosome (XXY), resulting in Klinefelter syndrome.

So, while a calico or tortoiseshell cat is very likely female, it's not impossible for a male cat to have this coat pattern.