- Root-knot nematode-infected roots
- Water
- Petri dish or small container
- Dissecting microscope or magnifying glass
Procedure:
1. Collect root-knot nematode-infected roots from a susceptible plant.
2. Place the roots in a petri dish or small container filled with water.
3. Cover the container and leave it at room temperature for several days.
4. Observe the roots under a dissecting microscope or magnifying glass. You should see small, white or transparent eggs attached to the roots.
5. After several days, the eggs will hatch and you will see small, motile nematodes swimming in the water.
Tips:
- To increase the chances of hatching, you can add a small amount of hydrogen peroxide to the water.
- You can also place the roots in a warm environment to speed up the hatching process.
- Once the eggs have hatched, you can transfer the nematodes to a new container and use them for further studies or experiments.