1. Safety First:
* Ventilation: Open windows and doors for good ventilation throughout the process.
* Gloves and Mask: Wear rubber gloves and a respirator mask to protect yourself from potentially harmful chemicals.
* Eye Protection: Use safety goggles to shield your eyes.
2. Assessing the Damage:
* Identify the Source: Locate the exact area where the urine soaked into the subfloor.
* Check for Mold: Look for signs of black mold or mildew, which can be a health hazard.
3. Removal and Cleaning:
Step 1: Thorough Drying:
* Absorbent Material: Use paper towels, old rags, or shop towels to soak up as much urine as possible.
* Blot, Don't Rub: Gently blot the area to avoid spreading the urine further.
* Air Dry: Allow the area to air dry completely before moving to the next step.
Step 2: Deodorizing and Cleaning:
* Enzyme Cleaner: Apply a pet-safe enzyme cleaner specifically designed for removing urine odor. These cleaners contain enzymes that break down the uric acid in urine, eliminating the odor.
* Hydrogen Peroxide (Optional): Mix a solution of 3% hydrogen peroxide with a small amount of dish soap. Apply it to the area, let it sit for 15 minutes, and then scrub with a brush. (Note: Hydrogen peroxide can bleach surfaces, so test it on an inconspicuous spot first).
* Baking Soda: Sprinkle baking soda over the affected area and let it sit for several hours or overnight. Then vacuum it up. Baking soda absorbs odors and helps neutralize them.
* Vinegar (Optional): White vinegar can also help neutralize odors. Dilute it with water (50/50 mix) and apply it to the area. Let it sit for a few minutes, then rinse thoroughly.
Step 3: Deep Cleaning and Drying:
* Carpet Cleaning Machine: Rent a carpet cleaning machine and use a pet odor-eliminating solution to clean the entire area, including the subfloor.
* Fan: Use a fan to circulate air and speed up drying.
4. Preventing Future Issues:
* Training: Train your dog to urinate and defecate in designated areas.
* Clean Up Accidents Quickly: Promptly clean up any accidents with a pet-safe cleaner to prevent odor and stains from setting in.
* Professional Cleaning: If you're dealing with a severe or persistent odor, consider consulting a professional carpet cleaner specializing in odor removal.
5. Mold Remediation:
* If you find mold: It's crucial to address it properly.
* Professional Help: Contact a professional mold remediation company. They will assess the situation and perform a safe and thorough mold removal.
Important Reminders:
* Ventilate Well: Ensure good ventilation throughout the cleaning process.
* Follow Instructions: Always follow the instructions on the cleaning products you use.
* Safety First: Wear appropriate protective gear to prevent chemical exposure.
* Patience: It may take multiple treatments to completely eliminate the odor.
By following these steps and using effective cleaning methods, you can remove dog urine odor from your subfloor and create a fresh-smelling home.