Can you charge a pet fee for at your hotel guide dog?

The answer is: no

According to the Americans with Disabilities Act (ADA), it is illegal to charge a pet fee for a service animal, such as a guide dog. Service animals are defined as dogs that are individually trained to do work or perform tasks for people with disabilities. This includes guiding people who are blind or visually impaired. Hotels and other businesses are required to allow service animals to accompany their handlers in all areas of the premises, without being charged a pet fee.