* Red Wolf: This critically endangered species was once found throughout the southeastern United States, but today only a few wild populations remain, primarily in eastern North Carolina.
* Eastern Cougar: Also known as the Florida panther, this large cat once roamed throughout the southeastern US but is now extremely rare in North Carolina.
* Piping Plover: A small shorebird that nests on beaches and sandbars along the Atlantic Coast, the piping plover is threatened by habitat loss and human disturbance.
* Loggerhead Sea Turtle: This marine turtle is found in warm ocean waters around the world and nests on beaches in North Carolina during the summer months. The loggerhead sea turtle is threatened by habitat loss, pollution, and fishing activities.
* Rafinesque's Big-eared Bat: A small, insectivorous bat found in caves and other suitable roosting sites in western North Carolina. The Rafinesque's big-eared bat is threatened by habitat loss and disturbance.
* Bog Turtle: A small, semi-aquatic turtle that inhabits sphagnum bogs and other wetlands in the coastal plain of North Carolina. The bog turtle is threatened by habitat destruction, pollution, and collection for the pet trade.
* Schweinitz's Sunflower: This rare plant is endemic to North Carolina and is found only in a few remaining pocosin wetlands. Schweinitz's sunflower is threatened by habitat loss and alteration.