Instructions
Note the temperature of water in the aquarium. Danio fish usually spawn at temperatures in the low to mid 70s Fahrenheit. If it is much cooler or warmer than this it is unlikely that your danio is carrying roe.
Make sure the fish you are looking at is actually a female. Male and female danios look very much the same, but if the fish in question turns out to be male he could actually be having some serious health problems. Female danios are generally fatter than male danios, whether they are carrying roe or not. In addition, in some species of danios, the females are slightly less colorful and may have fewer stripes, especially on the fins.
Look at your female danio. If she looks wider than normal, she is probably carrying roe. Since danios are not livebearers and female danios never carry fertilized eggs, this is as close as danios get to being pregnant. If your female is carrying roe, your male danio will fertilize the eggs as they are dropped, during spawning.
Watch your fishes' behavior. Danios like to spawn in the mornings. If your fish chase each other quite a bit at this time, you female may be carrying roe, and the fish may be spawning or near to spawning. In fact, if you see this type of near-violent behavior between male and female fish, your danios may have already spawned and produced eggs.
How to Know If a Danio Fish Is Pregnant
Danios are small schooling fish. There are many different breeds, with different colors and patterns, and many of these breeds are very popular with aquarium hobbyists. Although there are some types of fish that are hard to breed in an aquarium setting, danios breed well on their own. If you suspect your female danio may be pregnant, it's important to find out for sure, so that you can prepare the tank for the baby fish, called fry.