Do guppies lose their gravid spot after having her fry?

Yes, the gravid spot on a female guppy usually fades or disappears completely within 24 hours after giving birth. The gravid spot is a dark vertical line that runs along the belly of a pregnant guppy and indicates the presence of developing fry inside her. Once the fry are released, the gravid spot gradually becomes less noticeable and eventually vanishes, signaling that the female is no longer carrying any fertilized eggs.