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Are They Sisters?
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Check to make sure that you have two female bunnies. Put one bunny on her back to view the area at the base of her tail, placing a finger on either side of the genital area, and gently push down and apart. Both the male and female genitals will pop up. The female's will be smaller and shaped like a pyramid while the male's is more like a tube. If you cannot be sure, ask a veterinarian or someone experienced with rabbits.
Maintaining Sister Bunny Bond
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Give the sisters the best chance at continuing a close bond. Be wary of pet supply stores selling cages barely big enough to house one bunny, let alone two. The bunnies should be given a minimum of 8 square feet of cage and be let out at least four hours each day. Create an optimum atmosphere for the bunnies by litter training, and providing plenty of food, water and toys.
Spaying
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Even though the bunnies are sisters, spaying is still a highly recommended procedure. Having a veterinarian spay both bunnies at about 6 months old will prevent hormones from interfering later and, as an added bonus, keep the bunnies healthier. Bunnies that are not spayed could become aggressive and territorial, even with a litter mate. Watching over the sisters during the recovery time of 10 to 14 days is advised.
Getting to Know the Sisters
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Bunnies are just like people in that they have their own personalities and preferences. Spend enough time interacting with each bunny to learn what she is like. One sister may be lively and playful while the other is more laid back and quiet. Interpreting each bunny's temperament and needs keeps both happy.
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Can You Keep Two Female Sister Bunnies in the Same Cage?
When first acquiring bunnies as pets, many owners are faced with the question of how many should be kept together. While some people do not mind having male and female bunnies, most want to prevent the rabbits from multiplying by keeping two of the same sex. In this case, two female bunnies can be housed together, with sisters as the best option rather than pairing female bunnies from different litters.