When should the mom and dad bunny be separated after baby bunnies are born?

The doe (mother rabbit) and buck (father rabbit) should be separated immediately after mating or as soon as the babies (kits) are born, preferably before the doe gives birth. This is to prevent the buck from impregnating the doe again, which can lead to litter overlap, stress for the doe, and potential harm to the kits. Additionally, the buck's presence can cause stress and aggression towards the doe and the kits, so it's best to keep them separated. The doe will take care of the kits on her own and does not require the presence of the buck for raising them.